Anti-Centrin 1 Antibody, Affinity Purified (Rabbit Polyclonal)

Description

Centrin 1 (human; 172 aa, MW; 19,570) plays a fundamental role in microtubule organizing center structure and function. Centrins, also known as caltractins, are a family present in the centrioles and pericentriolar lattice. Human centrin genes are CETN1, CETN2 and CETN3. Centrins are required for duplication of centrioles. They may also play a role in severing of microtubules by causing calcium-mediated contraction.

Specifications:

Immunogen: Synthetic peptide conjugated to KLH, with a Cysteine residue linker corresponding to C terminal amino acids 155-168 (C-GEVNEEEFLRIMKK) of human centrin-1

Purity: Affinity-purified with the immunogen peptide

Form: 1mg/ml in PBS, 50% glycerol. Filter-sterilized. Azide and carrier free.

Reactivity: Reacts with human, mouse, rat, hamster and xenopus

Storage: Shipped at 4℃ and store at -20℃

Applications

Western blotting (~ 1/1,000 dilution)

Immunofluorescent staining (1/100~1/1,000 dilution)

Database Link:  UniProtKB Q12798 (CETN1_HUMAN). GeneCard CETN1.

Fig.1 Western blot analysis of endogenous centrin-1 in crude lysates. 1. HeLa cells (20μg); 2.MCF7 cells (20μg); 3.NIH3T3 cells (20μg); 4.Xenopus eggs (50μg). The anti-Centrin 1 antibody was used at 1/1,000 dilution.  Molecular mass is 19.6 kDa.

Fig.2. Immunofluorescence staining of centrin-1 protein in HeLa cells with anti-Centrin 1 antibody. Growing HeLa cells were fixed with 4% paraformaldehyde, and permeabilized with 0.25% Triton X-100. Anti-Centrin 1 antibody was used at 1/100 dilution. As 2^nd antibody, goat anti-rabbit IgG conjugated with Alex 488 (red) at 1/1,000 dilution. DNA was stained with DAPI (blue).