Cut15 protein is an essential component for DNA replication and also for the damage and checkpoint control which couples S and M phases (1, 2). It interacts with importin alpha (Imp1p), which together functions in nucleocytoplasmic transport and cell cycle progression. It contains 4 BRCT domains and the molecular mass is 60.4 kDa with 542 amino acids.
Western blot (100-1000 fold dilution)
Not tested for other applications
Immunogen: Recombinant GST-fusion protein with the full length of Cut15 protein
Specificity: Reacts with S. pombe Cut15 protein. Not tested for other species
Form: Rabbit antiserum with 0.05 % sodium azide
Storage: Shipped at 4 °C and store at -20°C.
UniProtKB/Swiss-Prot O14063 (IMA1_SCHPO)
References: This antibody was used in the following reference.
Matsusaka T et al “Mutations in fission yeast Cut15, an importin alpha homolog, lead to mitotic progression without chromosome condensation.” Curr. Biol. 8:1031-1034 (1998) PMID: 9740803
Umeda M et al “ The fission yeast Schizosaccharomyces pombe has two importin-alpha proteins, Imp1p and Cut15p, which have common and unique functions in nucleocytoplasmic transport and cell cycle progression” Genetics 171: 7-21 (2005) PMID:15937127
Figure 1. Identification of the Cut15 protein in the crude extract of S.pombe with this antibody.
Samples were prepared by alkali-lysis of the cells followed by TCA precipitation of proteins. S.pombe lysate 10 ug. Cut15 rabbit serum: 1000-fold dilution