Anti-DDX47 Antibody, Rabbit Serum

Description

DDX 47 gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene can shuttle between the nucleus and the cytoplasm, and has an RNA-independent ATPase activity. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

 

Applications

Western blot (~1,000 fold dilution)

Immunofluorescence staining (~500 fold dilution)

Other applications have not been tested.

 

 

 Specification

Reactivity: Reacts with DDX47 of human and rodents.

Immunogen: Purified Human DDX47 fused with GST

Form: Undiluted anti rabbit serum added with 0.05 % sodium azide

Storage: Shipped at 4°C, Upon arrival aliquot and store at -20°C

 

Data Link

UniProtKB/Swiss-Prot Q9H0S4 (DDX47_HUMAN)

GeneCards: DDX47 Gene

 

Reference This product was described and used in the following Ref.

 

Sekiguchi T et al “NOP132 is required for proper nucleolus localization of DEAD-box RNA helicase DDX47.” Nucleic. Acid. Res. 34 : 4593-4606 (2006) PMID: 16963496

 

 

Fig.1 Western blot analysis of DDX47 in the whole cell extracts of HeLa cells (10 μg) with anti-DDX47 antibody  at  1/1,000 dilution

 

Fig. 2 HeLa cells were fixed and immunostained with anti-DDX47 antibody followed by FITC-conjugated anti-rabbit IgG secondary antibody. DNA was stained with Hoechst dye.