Anti-DNA Polymerase β Antibody (Rabbit Antiserum)

$345.00

SKU: 70-041 Research Area: Applications:

Description

DNA polymerase β is a distributive polymerase involved in base excision repair which repairs damaged DNA by excising modified bases (oxidized, methylated, deaminated etc.). It has single-strand DNA binding and deoxyribose phosphodiesterase activities on the N-terminal side, and nucleotidyltransferase activity on the C-terminal side.  The enzyme is constitutively expressed in growing cells but the level of expression is further increased by treatment with alkylating reagents such as MNNG and MMS.

 

Specifications:

Reactivity: human, rat, mouse, hamster

Validated by full-size recombinant DNA polymerase β

Form: 1 mg/ml in PBS, 50% glycerol, filter-sterilized.  Azide and carrier free

Immunogen: Recombinant rat DNA polymerase beta, functional

Storage: Shipped at 4℃ and stored at -20℃

Continuation of 70-041 antiserum, this product was affinity-purified with immunogen.

 

Applications

Western blotting (1/ 2,000~ 1/3,000)

Immunoprecipitation (1/200)

Immunofluorescent staining (1/1,000)

ELISA (assay dependent)

 

Data Link

UniProtKB/Swiss-Prot P06766 (DPOLB_RAT), P06746 (DPOLB_HUMAN)

 

 

 

Fig.1 Detection of DNA polymerase beta in crude extract of HeLa cells by western blotting. The antibody was used at 1/2,000 dilution. 10 ug of the cell extract was used.

 

 

Fig.2 Detection of DNA polymerase beta by western blotting.

1.NIH 3TS cells (20 ug)

2.CHO cells (20 ug)

3.Full size recombinant DNA polymerase beta (5.2 ng)

The antibody was used at 1/2,000 dilution.

 

Fig.3 Immunofluorescence staining of DNA polymerase beta in NIH 3T3 cells with the antibody. The cells were fixed with 4% PFA and permeabilized with 0.25% Triton X-100. The anti-DNA polymerase beta antibody was used at 1/1,000 dilution. Nulear DNA was stained with DAPI and the merged image was shown on the right.

 

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