anti-E.coli RecA antibody, rabbit serum

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SKU: 61-003 Research Area: Applications:

Description

Description

  1. coli RecA protein (352 AA, 38 kD) plays critically important roles in homologous recombination, recombination repair and regulation of cellular responses to DNA damage (SOS response). RecA promotes auto-cleavage of LexA repressor by its co-protease activity after DNA damage, and induces many proteins related to DNA repair, including RecA itself (1).

 

Applications

  1. Western blot (1/3,000 dilution)
  2. Immunoprecipitation (1/600 dilution)
  3. Indirect immuno-fluorescent staining (assay dependent)
  4. ELISA (assay dependent)

 

Specification

Immunogen: Highly purified full-length recombinant E. coli RecA protein

Purity: The anti-serum was first adsorbed with extract of recA deletion strain and affinity-purified with purified RecA protein conjugated with agarose beads.

Form: Antibody solution at 1.0 mg/ml in PBS with 50% glycerol, filter-sterilized

Storage: Ship at 4°C or -20°C, and upon arrival, briefly centrifuge and store at -20°C.

 

Data Link  UniProtKB/Swiss-Prot  P0A7G6 (RECA_ECOLI)

 

References

  1. Friedberg EC et al. DNA Repair and Mutagenesis 2nd Ed. ASM Press
Title Type Size
61-003_03222018 application/pdf 276 KB

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