anti-E.coli RuvC antibody, rabbit serum

Description

E.coli RuvC protein is a structurally specific endonuclease which binds specifically to the Holliday structure, an intermediate of recombination, at the late stage of homologous recombination and recombination repair and introduces nicks at the symmetrical points of the Holliday junction, cleaving and resolving the recombinant (1). Its molecular weight is 19kD and forms a dimmer in liquid.

Applications

Western blot for the detection of E. coli RuvC protein x 3,000 dilution (Fig.1)

Other applications have not been tested.

Specification

Immunogen: Purified full-size recombinant RuvC protein (Ref. 2, 3)

Form: Rabbit antiserum with 0.05% sodium azide

Storage: 4°C for short term or -80°C for long term storage

Data Link

UniProtKB/Swiss-Prot P0A814 (RUVC_ECOLI)

References

This product was used in ref. 2 and 3.

1. Shinagawa H and Iwasaki H (1996) “Processing the holliday junction in homologous recombination” Trends Biochem Sci 21:107-111 PMID:
2. 8882584 Ichiyanagi K et al (1998) “Mutational analysis on structure-function relationship of a holliday junction specific endonuclease RuvC” Genes to Cells 3: 575-586 PMID: 9813108
3. Saito A et al (1995) “Identification of four acidic amino acids that constitute the catalytic center of the RuvC Holliday junction resolvase” Proc Natl Acad Sci USA 92: 7470-7474 PMID: 7638215
4. Friedberg EC et al DNA Repair and Mutagenesis 2^nd ed. ASM Press

Fig.1 Detection of RuvC (19kD) proteins in the cell extracts of E. coli carrying the plasmids which encode the indicated mutant RuvC proteins by Western blot using this antibody (Ref. 2).

WT: wild type RuvC protein

vector: vector plasmid

D7G, D7N, P8L: mutant RuvC proteins