Description
Background:
Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Higher plants also possess genes for significantly different, as yet uncharacterized Fd proteins, with extended C termini (FdCs). Whether these FdC proteins function as photosynthetic electron transfer proteins is not known. It has been suggested that FdC1 has a specific function in conditions of acceptor limitation at PSI, and channels electrons away from NADP(+) photoreduction.
Specifications:
Storage: Shipped at 4°C and store at -20℃
Form: 2 mg/ml in PBS, 50% glycerol. Filter sterilized. No preservative or carrier added.
Purity: IgG, affinity-purified with Protein A
Immunogen: Purified recombinant Arabidopsis Ferredoxin-C1 protein (full size, no tag attached)
Reactivity: Plant FdC1 proteins including that of maize and Arabidopsis.
Applications
- Western blot (1/1,000- 1/5,000 dilution)
- ELISA (assay dependent)
Fig. 1 Western Blot of Fdx2 protein.
Anti-FdC1 antibody was used at 1/1,000 dilution. Second antibody (goat anti-rabbit IgG antibody HRP-conjugated, ab97051) was used at 1/10,000 dilution.
- Arabidopsis leaf extract, 10 μg
- Maize leaf extract, 10 μg
Molecular mass of arabidopsis FdC1 is 16.7 kDa
