Description
Background:
Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. It occupies a key position both for transferring the photoreducing power to Fd-NADP+ oxidoreductase (FNR), hence the formation of NADPH, and for mediating the cyclic electron flow around photosystem I (PSI).
Subcellular location: Chloroplast
Specifications:
Storage: Shipped at 4°C and store at -20℃.
Form: 1 mg/ml in PBS, 50% glycerol. Filter sterilized. No preservative or carrier added.
Purity: IgG, affinity-purified with protein A agarose.
Immunogen: Purified recombinant maize Fd1 protein (full size, no tag attached)
Reactivity: Reacts with plant Fd1 and Fd2 isoproteins including those of Maize and Arabidopsis
Validation: Specificity has been validated by WB with purified maize Ferredoxin-1 (Fd1) protein.
Applications
- Western blotting (1/1,000- 1/5,000 dilution)
- ELISA (assay dependent)
Fig.1 Western Blot of Ferredoxin isoproteins with anti-Ferredoxin-1 (maize) antibody in plant leaf extracts.
Anti-Fd1 antibody was used at 1/1,000 dilution. Secondary antibody (goat anti-rabbit IgG antibody HRP-conjugated, ab97051) was used at 1/10,000 dilution.
- Recombinant Maize Fd1.
- Arabidopsis leaf extract, 10 μg
- Maize leaf extract, 10 μg
Molecular mass of Maize Fds are about 12kDa, but migrates at the position around 15 kDa on SDS-PAGE.
