Description
Background:
Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Occupies a key position both for transferring the photoreducing power to Fd-NADP+ oxidoreductase (FNR), hence the formation of NADPH, and for mediating the cyclic electron flow around photosystem I (PSI).Fd2 is most abundant Fd isoproteins expressed in plant leaves.
Subcellular location: Chloroplast and Plastid
Specifications:
Storage: Shipped at 4°C and store at -20℃
Form: 2 mg/ml in PBS, 50% glycerol. Filter sterilized. No preservative or carrier added.
Purity: IgG, affinity-purified with Protein A
Immunogen: Purified recombinant Arabidopsis Fd2 protein (full size, no tag attached)
Reactivity: Plant Fd2 proteins including that of maize and Arabidopsis.
Validation: Specificity has been validated by Western blot with recombinant Arabidopsis Ferredoxin-2 (Fd2) protein.
Applications
- Western blot (1/1,000- 1/5,000 dilution)
- ELISA (assay dependent)
Fig. 1 Western Blot of Fd2 protein with anti-Ferredoxin-2 (arabidopsis) antibody.
Anti-Fdx2 antibody was used at 1/1,000 dilution. Secondary antibody (goat anti-rabbit IgG antibody HRP-conjugated, ab97051) was used at 1/10,000 dilution.
- Arabidopsis leaf extract, 10 μg
- Maize leaf extract, 10 μg