anti-Ferredoxin-2 (plant) antibody, rabbit polyclonal 100 ug

$380.00

SKU: 81-017 Research Area: Applications: ,

Description

Background:

Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Occupies a key position both for transferring the photoreducing power to Fd-NADP+ oxidoreductase (FNR), hence the formation of NADPH, and for mediating the cyclic electron flow around photosystem I (PSI).Fd2 is most abundant Fd isoproteins expressed in plant leaves.

 

Subcellular location: Chloroplast and Plastid

 

Specifications:

Storage: Shipped at 4°C and store at -20℃

Form: 2 mg/ml in PBS, 50% glycerol. Filter sterilized. No preservative or carrier added.

Purity: IgG, affinity-purified with Protein A

Immunogen: Purified recombinant Arabidopsis Fd2 protein (full size, no tag attached)

Reactivity: Plant Fd2 proteins including that of maize and Arabidopsis.

Validation: Specificity has been validated by Western blot with recombinant Arabidopsis Ferredoxin-2 (Fd2) protein.

 

Applications

  1. Western blot (1/1,000- 1/5,000 dilution)
  2. ELISA (assay dependent)

Fig. 1 Western Blot of Fd2 protein with anti-Ferredoxin-2 (arabidopsis) antibody.

Anti-Fdx2 antibody was used at 1/1,000 dilution. Secondary antibody (goat anti-rabbit IgG antibody HRP-conjugated, ab97051) was used at 1/10,000 dilution.

  1. Arabidopsis leaf extract, 10 μg
  2. Maize leaf extract, 10 μg
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81-017 anti-Ferredoxin-2 (plant) antibody application/pdf 275 KB