Description
Background:
Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Occupies a key position both for transferring the photoreducing power to Fd-NADP+ oxidoreductase (FNR), hence the formation of NADPH, and for mediating the cyclic electron flow around photosystem I (PSI).
Specifications:
Storage: Shipped at 4°C and store at -20℃
Form: 2 mg/ml in PBS, 50% glycerol. Filter sterilized. Azide and carrier free
Purity: IgG, affinity-purified with Protein A
Immunogen: A mixture of four Maize Ferredoxin isoproteins, Fd1, Fd2, Fd3 and Fd4
Reactivity: Essentially all plant Ferredoxin (Fd) isoproteins including those of Arabidopsis and Maize
Applications
- Western blot (1/1,000- 1/10,000 dilution)
- ELISA (assay dependent)
Fig.1 Western Blot of Fd in plant leaf extract.
Anti-Fd antiserum was used at 1/1,000 dilution. Secondary antibody (goat anti-rabbit IgG antibody HRP-conjugated, ab97051) was used at 1/10,000 dilution.
- Arabidopsis leaf extract, 10 μg
- Maize leaf extract, 10 μg
Molecular masses of Fd isoproteins are about 12 kDa, but they migrate at the position around 16-17 kDa on the SDS-PAGE gel due to their strong acidic nature.
