Description
Background:
Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.
Subcellular location: Apicoplast (plastid-like organelle)
Specifications:
Storage: Shipped at 4°C and store at -20˚C
Form: 4 mg/ml in PBS, 50% glycerol. Filter sterilized. No preservative or carrier added.
Purity: IgG, affinity-purified with Protein A
Immunogen: Ferredoxin (Fd) protein purified from Malaria parasite, Plasmodium falciparum.
Reactivity: Plasmodium falciparum Ferredoxin
Applications
- Western blot (1/500- 1/2,000 dilution)
- ELISA (assay dependent)
- Immunofluorescent staining (assay dependent)
Fig.1 Western Blot of P. falciparum. Ferredoxin.
Anti-Pf Fd antibody was used at 1/1,000 dilution. Secondary antibody (goat anti-rabbit IgG antibody HRP-conjugated) was used
1.Purified recombinant Pf Ferrodoxin. 10 ng
2.Partially purified Pf Ferredoxin from culture of
- falciparum.
- Purified recombinant Pf Ferrodoxin. 1.4 ng
Molecular mass of Pf Fd is 18 kDa
Fig. 2 Immunofluorescence staining of ferredoxin in P. falciparum.
Trophozoite and schizont stages of P. falciparum were stained with the anti- Pf Fd antibody (right panels, red color). Nuclear DNA was stained with DAPI (middle panels, blue color). Dark spots in bright field microscopy (left panels) are hemozoin pigment.
