Description
Background:
Fd:NADPH oxidoreductase (FNR) plays a key role in regulating the relative amounts of cyclic and non-cyclic electron flow to meet the demands of the plant for ATP and reducing power. The human malaria parasite (Plasmodium falciparum) possesses a plastid-derived organelle called the apicoplast, which is believed to employ metabolisms crucial for the parasite’s survival.
Subcellular location: Apicoplast (plastid-like organelle)
Specifications:
Storage: Shipped at 4°C and store at -20℃.
Form: 4 mg/ml in PBS, 50% glycerol. Filter sterilized. Azide and carrier free
Purity: IgG, affinity-purified with protein A agarose.
Immunogen: Purified recombinant P. falciparum Ferredoxin-NADP reductase (full-size, no-tag attached) expressed in E. coli.
Reactivity: FNR protein of Plasmodium falciparum. Cross-reacts also with plant FNR isoproteins.
Applications
- Western blot (1/500-1/2,000 dilution). Extract for Western blot should be made from apicoplast fraction of falciparum.
- ELISA (assay dependent)
Fig.1 Western blot of Ferredoxin–NADP reductase of P. falciparum with anti-Pf FNR antibody.
Anti-Pf FNR antibody was used at 1/1,000 dilution. Second antibody (goat anti-rabbit IgG antibody HRP-conjugated, ab97051) was used at 1/10,000 dilution.
Sample: 1 µl of 40 µM recombinant pf FNR
Molecular mass indicated from the gene is 43.8 kDa. However, transit peptide consisting of N-terminal 18 amino acids is removed in the mature form.
