anti-GFP antibody, rabbit serum

Description

The green fluorescent protein (GFP) is composed of 238 amino acids (26.9 kDa), originally isolated from the jellyfish Aequorea victoria that fluoresces green when exposed to blue light (1). In cell and molecular biology, the GFP fused gene is frequently used as a reporter of expression and protein localization (2, 3).

Applications

1. Western blot 1/2,000 dilution 2. Immunoprecipitation (assay dependent)
2. Immunohistochemistry 1/4,000 dilution 4. Immunofluorescence 1/4,000 dilution

Other applications were not tested

Specification

Immunogen: Recombinant His-tagged EGFP

Reactive to all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, EGFP, and their-fusion proteins

Form: Antiserum with 0.05% sodium azide

Storage: Shipped at 4°C or -20°C. Upon arrival, aliquot and store at -20°C

Data Link

Swiss-Prot P42212 (GFP_AEQVI)

References

1. Shimomura O et al (1962) “Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea.” J Cell Comp Physiol 59: 223–239 PMID: 13911999

2. Chalfie M et al (1994) “Green fluorescent protein as a marker for gene expression.” Science 263 (5148): 802–805 PMID: 8303295

3. Tsien R (1998) “The green fluorescent protein.” (PDF) Annu Rev Biochem 67: 509–544 PMID: 9759496