anti-HCV NS4a protein antibody, monoclonal, FITC conjugated

$310.00

SKU: 65-059 Research Area: , Applications:

Description

Hepatitis C virus (HCV) is a small (55-65 nm in size), enveloped, positive sense single-stranded RNA virus in the family Flaviviridae and the principal cause of parenteral non-A, non-B hepatitis. The virus genome consists of a single open reading frame of approximately 9,400 bases which encodes a single polyprotein of about 3,010 amino acids (1, 2, 3). The polyprotein is processed by host cell and viral proteases into four structural proteins (core, envelope1 and 2, and p7) and six non-structural proteins (NS2, 3, 4a, 4b, 5a, and 5b) necessary for viral replication. NS3 serine proteinase is responsible for proteolytic processing of other non-structural proteins. NS4a protein (54 amino acids) forms a complex with NS3 and functions as a cofactor for NS3 protease activity.

This product is an FITC-labeled IgG ([FITC]/[IgG] = 10.4) produced from the IgG fraction.

 

Applications:

  1. Western blot
  2. Immunofluorescence staining
  3. ELISA
  4. FACS

 

Specification:

Immunogen: A region of NS4 protein (the nucleotide sequence is shown in ref.4) of HCV genotype 1b expressed in E. coli. The epitope of this antibody was mapped to the N-terminal region of the NS4 protein (NS4a).

Isotype: Mouse IgG2b kappa

Form: Purified monoclonal antibody (IgG) 1.4 mg/ml in PBS, 50% glycerol, filter-sterilized

Specificity: Specific to human HCV NS4a protein

Storage: -20°C

 

Data Link: UniProtKB HCV protein

 

References: This antibody is produced and used in ref. 4.

  1. Brass V et al (2006) Molecular Virology of Hepatitis C Virus (HCV): 2006 Update Int J Med Sci 3:29-34 PMID: 16614739
  2. Kato N et al (1990) “Molecular cloning of the human hepatitis C virus genome from Japanese patients with non-A, non-B hepatitis” Proc Natl Acad Sci USA 87: 9524-9528 PMID: 2175903
  3. Takamizawa A et al (1991) “Structure and organization of the hepatitis C virus genome isolated from human carriers” J Virol 65: 1105-1113 PMID: 1847440
  4. Manabe S et al (1994) “Production of nonstructural proteins of hepatitis C virus requires a putative viral protease encoded by N3” Virology 198: 636-644 PMID: 8291245

Fig. 1. Western blotting of HCV NS4a protein.
Chimp liver cells were infected with recombinant vaccinia virus containing a HCV genome cDNA and were subjected to Western blotting using anti-NS4a antibody. The protein detected with this antibody is 6 kD. This small NS4 protein (NS4a) was produced from the N-terminal region of the NS4 protein.

Fig. 1. Western blotting of HCV NS4a protein.
Chimp liver cells were infected with recombinant vaccinia virus containing a HCV genome cDNA and were subjected to Western blotting using anti-NS4a antibody. The protein detected with this antibody is 6 kD. This small NS4 protein (NS4a) was produced from the N-terminal region of the NS4 protein.

Title Type Size
65-059 Anti-Hepatitis C virus NS4a_03232018 application/pdf 208 KB

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