Description
Background:
Ferredoxin-NADP reductase, leaf isozyme 3 (L-FNR3) plays a key role in regulating the relative amounts of cyclic and non-cyclic electron flow to meet the demands of the plant for ATP and reducing power.
Specifications:
Storage: Shipped at 4°C and store at -20℃.
Form: 1 mg/ml in PBS, 50% glycerol. Filter sterilized. No preservative or carrier added.
Purity: IgG, affinity-purified with protein A agarose from rabbit antiserum
Immunogen: Purified recombinant maize leaf FNR3 protein (full size, no tag attached)
Reactivity: Plant L-FNR proteins including Maize L-FNR3, L-FNR2 and L-FNR1, and Arabidopsis FNR1 and FNR2 in the order of reactivity in each species.
Validation: Specificity has been validated by WB with recombinant full-size L-FNR3
Applications
- Western blotting (1/1,000- 1/10,000 dilution)
- ELISA (assay dependent)
Other applications have not been tested.
Fig.1 Western blot detecton of L-FNR isoproteins in plant leaf extracts with anti-L-FNR3 antibody..
Anti-L-FNR3 antiserum was used at 1/1,000 dilution. Second antibody (goat anti-rabbit IgG antibody HRP-conjugated, ab97051) was used at 1/10,000 dilution.
- Arabidopsis leaf extract, 2 μg
- Maize leaf extract, 2 μg
The antibody reacts with L-FNR3 and other L-FNR isoproteins in Maize and Arabidopsisleaf extracts.
The molecular masses of mature forms of maize FNR1, FNR2 and FNR3 are 34.97, 35.57 and 34.7 kD, respectively (Ref 1)
