Anti-NADase (hemolytic streptococcus) antibody, rabbit serum

Description

NAD (nicotinamide adenine dinucleotide) hydrolyzing enzyme is one of the extracellular enzymes and toxins produced by hemolytic streptococci. Although the function as a toxin is largely unknown, it has been suggested to be related to pathogenicity of acute infection (1).  NADase is produced not only by Group A hemolytic streptococci but also by Group C and Group G strains. The amino acid sequences are highly conserved among them and the antibodies cross-react each other. Upon infection of hemolytic streptococci, the antibody titer to the NADase is increased similarly to anti-SLO (Streptolysin O) antibody.

The product was prepared by immunizing rabbit with purified recombinant NADase of Group C hemolytic streptococci expressed in E. coli (2).

Application

• Western blot (x2,000~10,000 dilution)
• Immunoprecipitation
• Neutralization of NADase activity

Specifications

Immunogen: Purified recombinant NADase of Group C hemolytic streptococci expressed in E. coli

Form: Undiluted antiserum with 0.09 % sodium azide                                

Reactivity: NADase of Group A, C, and G origins

Storage: Ship 4^°C, store -20°

Data Link  UniProtKB/TrEMBLQ5R2E3 (Q5R2E3_STREQ)

Reference

1. Minami M et al. “Clindamycin-Induced CovS-Mediated Regulation of the Production of Virulent Exoproteins Streptolysin O, NAD Glycohydrolase, and Streptokinase in Streptococcus pyogenes”Antimicrob.AgentsChemother.49:88–96(2010)..PMID: 19805566

2. H. Kimoto et al.: Genetic and biochemical properties of streptococcal NAD-glycohydrolase inhibitor. J. Biol. Chem., 277, 14695-14702 (2006). PMID: 16380378

Fig. Detection of NADase in the culture supernatant of hemolytic streptococci with anti-NADase.

Lane 1: Culture supernatant of group A streptococcus

Lane 2: Culture supernatant of group C streptococcus

Lane 3: Culture medium only (negative control)

* This product is for research use only, not for human use