Anti-Rad18 (human) antibody, rabbit polyclonal, affinity purified

Description

The Rad6-Rad18 pair of genes plays a critical role in post-replication repair of damaged DNA. Rad6 protein functions as an E2 enzyme and Rad18 (495 aa 56 kDa) as an ubiquitine ligase (E3) which ubiquitinates PCNA. Rad18 recruits translesion DNA polymerases to damaged DNA (ref 1~3).

Applications see Ref 1~3

1) Western blot (1,000 fold dilution)

2) Indirect immunofluorescence staining

3) Immunoprecipitation

Specification

Immunogen: GST-fusion protein containing 113 carboxyl terminal of human Rad18

Reactivity: Human Rad18. Not reactive to mouse Rad18

Purification: Polyclonal rabbit IgG fraction affinity purified with His-tagged Rad18 protein affinity column

Concentration: 1 mg/m in PBS, 50% glycerol

Storage: Ship at 4°C or at -20°C, and long term storage at -20°C

Data Link UniProtKB; E3 ubiquitin-protein ligase RAD18 – Homo sapiens (Human)

References:  This product has been used in the following references.

1. Miyase S et al “Differential regulation of Rad18 through Rad6-dependent mono- and polyubiquitination” J Biol Chem 280: 515-524 (2005) PMID: 15509568
2. Tateishi S et al “Dysfunction of human Rad18 results in defective postreplication repair and hypersensitivity to multiple mutagens” PNAS 97: 7927-7932 (2000) PMID: 10884424
3. Watanabe K et al “Rad18 guides poleta to replication stalling sites through physical interaction and PCNA monoubiquitination” EMBO J 23: 3886-3896 (2004) PMID: 15359278

Fig. 1 Indirect immunofluorescence staining of Rad18 protein in GM637 cells.
Rad18 protein is stained as yellow dots in nuclei.

Fig. 2 Identification of Rad18 protein in crude extract of A549 cells by Western blot.
The lower thick band is native Rad18 and the upper thin band is mono-ubiquitinated Rad18　protein (ref. 1)