Anti-Rad6 (human) antibody, affinity purified (rabbit polyclonal)

Description

Resuming of DNA synthesis, which was halted by various physical and chemical factors, was triggered by repair system including Rad6. Rad6 (152 aa, 17.3kDa) is the ubiquitin-conjugated enzyme E2A, and complex with two Rad18 molecules, ubiquitin ligase E3, to ubiquitinate PCNA by utilizing poly-ubiquitin chain that HLTF-UBC13 synthesized in translesion DNA synthesis (TLS) and template switch (TS). Two isoforms of Rad6, Rad6A and Rad6B differs in only 6 amino acids. It also plays an important role in epigenetic transcriptional regulation by catalyzing the monoubiquitination of histone H2B at Lys-120.

Applications

• Western blot (1,000~2,000 fold dilution)
• Immunofluorescent staining (assay dependent)
• Immunoprecipitation (assay dependent)

Specification

Reactivity: Reacts with Rad6A and Rad6B of human, mouse, rat and hamster.

Immunogen: Human Rad6B full-length recombinant protein

Purity: Affinity purified with the full-length human Rad6A protein

Form: 1 mg/ml in PBS, 50% glycerol, filter-sterilized. Sodium azide and carrier-free.

Storage: Ship at 4°C. Upon arrival centrifuge, aliquot and store at -20°C.

Data Link: UniProtKB/Swiss　P49459　(UBE2A/Rad6A_HUMAN)

UniProtKB/Swiss　P63146  (UBE2B/Rad6B_HUMAN)

Reference: This product was used in the following reference.

Masuyama S. et al. “Regulated expression and dynamic changes in subnuclear localization of mammalian Rad18 under normal and genotoxic conditions” Genes to Cells 10: 753-762 (2005) PubMed 16098139

Fig. 1 Western blot analysis of Rad6 in the whole cell extracts of HeLa cell (10 μg) with anti-Rad6 antibody (1/1,000 dilution)

Fig. 2 Rad6 protein in GM637 cells were stained by indirect immunostaining with the Rad6 antibody