Anti-Sds22 (S. pombe) antibody, rabbit serum

Description

Schizosaccharomyces pombe Sds22 protein contains leucine-rich repeats and physically interacts with the catalytic subunits of two type 1 protein phosphatases (Dis2 and Sds21). Sds22 is a regulatory subunit of these phosphatases and the Sds22-bound phosphatases carry a key phosphatase activity essential for the progression from metaphase to anaphase. Sds22 is essential for cell viability and in its absence, cells were blocked in metaphase. Sds22 protein is predicted to form a repeating helical rod that is capable of enhancing a PP1-dependent dephosphorylation activity.

Applications

1. Immunoblot (dilution: 1/200~1/500) (Ref 1,2,3)
2. Immunoprecipitation (Ref 2)
3. Immunofluorescence microscopy

Specifications

Immunogen: Recombinant C-terminal region (1.8kb) of S. pombe Sds22 (1)

Specificity: Specific to S. pombe

Form: Rabbit antiserum with 0.05 % sodium azide

Storage: Ship at 4°C and long term storage at -20°C

Data Link

Swiss-Prot P22194

References:

 This antibody has been used in Ref. 1, 2 and 3.

1. Ohkura H and Yanagida M “S. pombe gene sds22 essential for a midmitotic transition encodes a leucine-rich repeat protein that positively modulates protein phosphatase-1.” Cell 64: 149-157 (1991) PMID: 1846086
2. Stone EM et al “Mitotic regulation of protein phosphatases by the fission yeast sds22 protein.” Curr Biol 3: 13-26 (1993) PMID: 15335873
3. Ishii K et al “Requirement for PP1 phosphatase and 20S cyclosome/APC for the onset of anaphase is lessened by the dosage increase of a novel gene sds23 .” EMBO J. 15:6629-6640 (1996) PMID: 8978689

Fig.1 Immunoblot with anti-Sds22 antiserum of yeast extracts from (1) wild type strain HM123, (2) sds::ura4 deletion mutant carrying pHR140-2 (ref.2).

The 40kD protein band was identified by immunoblot analysis of wild-type strain using anti-Sds22 antisera (lane1). The 40 kD band is enhanced in the sds22::ura4 disruption mutant strain that is rescued by the multicopy sds22 plasmid pHR140-2 (lane2).