Description
Background:
May degrade glucosinolates (glucose residue linked by a thioglucoside bound to an amino acid derivative) to glucose, sulfate and any of the products: thiocyanates, isothiocyanates, nitriles, epithionitriles or oxazolidine-2-thiones. These toxic degradation products can deter insect herbivores. Seems to function in abscisic acid (ABA) and methyl jasmonate (MeJA) signaling in guard cells. Functionally redundant with TGG1.
Subcellular location: Vacuole
Specifications:
Storage: Shipped at 4°C and store at -20℃
Form: 1 mg/ml in PBS, 50% glycerol. Filter sterilized. No preservative or carrier added.
Purity: IgG, affinity-purified with Protein A/G mix
Immunogen: A synthetic peptide, AHALDPSPPEKLT, corresponding to TGG2 protein (363-376)) of A. thaliana, conjugated with bovine serum albumin. This region of TGG2 has little homology with the corresponding region of TGG1.
Reactivity: React with TGG2 of Arabidopsis but does not react with TGG1 of Arabidopsis.
Validation: Specific reactivity has been validated by Western blot showing that the TGG2 specific band is absent in tgg2-1 mutant leaf extract (Ref.1)
Applications
- Western blot (1/1,000 dilution)
- ELISA (assay dependent)
- Immunoelectron microscopic analysis (assay dependent)
Fig. 1 Western blot of TGG2 in crude extract of Arabidopsis leaves.
Blotted in wet system overnight. The anti-TGG2 antibody was used at 1/1,000 dilution.
Molecular mass calculated from the amino acid sequence is 63 kDa, Signal peptide of 28 amino acids is removed and three glycosylation sites have been identified in the mature form.