Anti-U1 snRNP C (U1C) antibody, rat monoclonal (4H12)

Description

The spliceosomal U1C protein is critical to the initiation and regulation of precursor messenger RNA (pre-mRNA) splicing, as part of the U1 small nuclear ribonucleoprotein particle (snRNP). U1C is needed for efficient complex formation of U1 snRNP with a 5’ splice site. The antibody was produced from the hybridoma cultured in serum-free medium and purified under mild conditions by propriety chromatography processes.

Applications

1. Western blot Immunocytochemistry 3. Immunohistochemistry (frozen section)
2. ELISA Other applications have not been tested.

Specifications

Immunogen: Recombinant GST-fused mouse U1C full length

Isotype: Rat IgG 2a kappa

Form: Purified monoclonal antibody (IgG) 1mg/ml in PBS, 50% glycerol, filter-sterilized

Specificity: Specific to human, simian, and mouse U1C. Other species have not been tested.

Storage: -20°C, for long term storage -70°C

Data Link UniProtKB/Swiss-Prot Q62241 (RU1C_MOUSE)

References

1. Muto Y et al “The structure and biochemical properties of the human spliceosomal protein U1C” J Mol Biol 341: 185–198 (2004) PMID: 15312772
2. Pomeranz Krummel DA et al “Crystal structure of human spliceosomal U1 snRNP at 5.5A resolution” Nature 458: 475-480 (2009) PMID: 19325628