anti-yeast Srb4p antibody, rabbit serum

Description

Background:

Srb4 protein is subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for basal RNA polymerase II transcription; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination

Specifications:

Reactivity: S. cerevisiae Srb4 protein.

Immunogen: Recombinant full-size yeast His-tagged Srb4 protein (1-687 aa)

Form: Whole antiserum added with 0.1% sodium azide.

Storage: Ship at 4℃ and store at -20℃.

Applications:

• Western blotting (1/1,000~1/5,000 dilution)
• Immunoprecipitation (assay dependent)
• Chromatin Immunoprecipitation (assay dependent)

Data Link: SGD SRB4/YER022W S. cerevisiae Srb4 protein   SwissProt: P32569 S. cerevisiae   Entrez Gene: 856743 Saccharomyces cerevisiae

Fig.1. Detection of Srb4p in crude extract of S. cerevisiae by Western blotting

Extract of S. cervisiae strain BY4741 (10 μg) was used. Tahe antibody was used at 1/1,000 dilution. IMMUNO SHOT (CosmoBio, Tokyo) was used as signal enhancer.

Molecular mass of Srb4 protein is 78.5 kDa.