DNA polymerase Beta (Rat)

$315.00$945.00

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SKU: 10-101 Research Area: Applications:

Description

DNA polymerase is a distributive polymerase involved in base excision repair which repairs damaged DNA by excising modified bases (oxidized, methylated, deaminated etc.) (ref. 1).

This product is highly purified full-length rat DNA polymerase overproduced in E. coli with high enzymatic activity without any tag attached (ref. 2). The enzyme has molecular mass of 38 kDa (Fig.1). The amino acid sequence of the rat enzyme has 86% identity to the human homolog.

 

Applications

  • For the studies on the mechanisms of base-excision repair of DNA damage

2)  As a positive control for Western blot with anti-DNA polymerase antibody

 

Specification

Enzyme activity: 90 unit/μl (1 unit of the enzyme activity incorporates 1 nanomole of dNTP into acid-insoluble fraction at 37 in 60 minute)

Purity: Over 95% purity by SDS-PAGE analysis

Form: 1.3 mg/ml in 50mM Tris-HCl pH7.6, 0.3M KCl, 0.1mM EDTA, 1mM DTT, 20% glycerol

Storage: -20°C or for long term storage, – 70°C

 

Data Link

UniProtKB/Swiss-Prot P06766 (DPOLB_RAT)

 

References

  1. Friedberg EC et al DNA Repair and Mutagenesis 2nd,ASM Press (2006)
  2. Date T et al “Expression of active rat DNA polymerase
    beta in Escherichia coli.” Biochemistry 27: 2983-2990 (1988) PMID: 3042024

Fig.1 SDS-PAGE analysis of DNA polymerase β

M: Molecular weight markers (from top: 250, 150, 100, 75, 50, 37, 25, 20 kDa)

Lane1: DNA polymerase b (rat)

Title Type Size
10-101_03222018 application/pdf 281 KB

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