DNA polymerase kappa (human, C-His tag), functional

$490.00

SKU: 10-105 Research Area: Applications:

Description

Mammalian DNA polymerase ĸ, a member of the UmuC/DinB nucleotidyl transferase superfamily, has been implicated in spontaneous mutagenesis (Ref.1). Human DNA polymerase ĸ copies undamaged DNA with average single-base substitution and deletion error rates of 7 x 10-3 and 2 x 10-3, respectively. These error rates are high when compared to those of most other DNA polymerases (Ref.2). DNA polymerase ĸ has important role in the mutagenic bypass of certain types of DNA lesions (Ref.3).

 

This product was over-expressed as a recombinant protein in E. coli with a plasmid carrying a C-terminal histidine-tagged human DNA polymerase ĸ (1-560 aa), and highly purified by several steps of chromatography (Ref.2). The product is catalytically active and its molecular weight is 65 kD (Fig 1). Activity of this product has been confirmed at dilutions as high as 8,000-fold.

 

Application:

  • Functional studies as translesion DNA synthesis by DNA polymerase kappa
  • Antigen for Western blot and ELISA by anti-Pol Kappa antibody
  • SDS-PAGE

 

Specification:

Purity: Over 90% by SDS-PAGE (CBB staining)

Protein concentration: 3.2 mg/ml as measured by BCA method

Form:  0.2 M NaCl, 10 mM sodium phosphate buffer (pH 7.0), 50% glycerol

Storage: Ship at 4°C or -20°C and upon arrival briefly centrifuge and store at -20°C or at -80°C for longer storage.

 

Data Link: Swiss-Prot Q9UBT6

 

References This product has been used in the following publications.

  1. Ohashi E et al “Fidelity and processivity of DNA synthesis by DNA polymerase kappa, the product of the human DINB1 gene” J Biol Chem 275: 39678-39684 (2000) PMID: 11006276 Function
  2. Ohashi E et al “Error-prone bypass of certain DNA lesions by the human DNA polymerase kappa” Genes Dev 14: 1589-1594 (2000) PMID: 10887153 Function
  3. Jałoszyński P. et al (2005) Error-prone and inefficient replication across 8-hydroxyguanine (8-oxoguanine) in human and mouse ras gene. Genes Cells.10:543-50. PMID:15938713
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10-105_03222018 application/pdf 286 KB

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