E. coli RecQ helicase contributes to the genomic stability as the prototype of RecQ family DNA helicases, mutations of which genes are associated with premature aging and cancer susceptibility, known as Bloom’s and Werner’s syndromes (1).
Product: Highly purified (≥95%) recombinant full-length E. coli RecQ protein.
Form: 20 mM Tris-HCl (pH 7.5), 1 mM EDTA, 50 mM KCl, 1 mM DTT, 10% glycerol,
Purity: Over 90% by SDS-PAGE (CBB staining)
Protein concentration: 0.5 mg/ml as measured by BCA method
Biochemical activities: Unwinding duplex DNA, dependent on ATP. DNA-dependent ATPase (Ref.2).
Storage: Ship at 4℃ or at -20℃ and upon arrival, centrifuge briefly and store at -20℃ or -80℃ for longer period
- DNA dependent ATPase
- DNA helicase specific for various form
of DNA structure like branched form and forlk.
|Figure. SDS-PAGE analysis of the purified RecQ protein|
Data Link UniProtKB/Swiss-Prot P15043 (RECQ_ECOLI)
References: This product was used in Ref 2