E. coli single-stranded DNA binding protein (SSB) binds to single-stranded DNA with high specificity (1, 2). It is involved in DNA replication and recombination in vivo. The SSB gene was expressed as the recombinant protein in E. coli then purified. The molecular mass is 18.9 kDa.
- Functional single-stranded DNA-binding protein for studying DNA replication and recombination
- Enhancement of the specificity and yield of PCR
Quality: Greater than 95% purity as determined by SDS-PAGE (CBB staining). The absence of endonucleases and exonucleases was confirmed.
Form: 5 mg/ml in 20mM Tris-HCl (pH 7.6), 200mM NaCl, 1mM dithiothreitol, 1mM EDTA, 50% glycerol
Data Link: UniProtKB/Swiss-Prot P0AGE0 (SSB_ECOLI)
- Krauss G et al (1981) “Escherichia coli single-strand deoxyribonucleic acid binding protein: Stability, specificity, and kinetics of complexes with oligonucleotides and deoxyribonucleic acid.” Biochemistry 20: 5346-5352 PMID: 7028102
- Weiner JH et al (1975) “The deoxyribonucleic acid unwinding protein of Escherichia coli. Properties and functions in replication.” J. Biol. Chem. 250:1972-1980 PMID: 1090613
Fig. 1 SDS-PAGE of E. coli SSB protein
Fig. 2 Binding activity to single-stranded DNA