E. coli SSB (Single-Stranded DNA Binding) protein

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SKU: 02-042 Research Area: Applications:

Description

E. coli single-stranded DNA binding protein (SSB) binds to single-stranded DNA with high specificity (1, 2). It is involved in DNA replication and recombination in vivo. The SSB gene was expressed as the recombinant protein in E. coli then purified. The molecular mass is 18.9 kDa.

 

Applications

  1. Functional single-stranded DNA-binding protein for studying DNA replication and recombination
  2. Enhancement of the specificity and yield of PCR

Specification

Quality: Greater than 95% purity as determined by SDS-PAGE (CBB staining). The absence of endonucleases and exonucleases was confirmed.

Form: 5 mg/ml in 20mM Tris-HCl (pH 7.6), 200mM NaCl, 1mM dithiothreitol, 1mM EDTA, 50% glycerol

Storage: -20°C

 

Data Link: UniProtKB/Swiss-Prot P0AGE0 (SSB_ECOLI)

 

References:

  1. Krauss G et al (1981) “Escherichia coli single-strand deoxyribonucleic acid binding protein: Stability, specificity, and kinetics of complexes with oligonucleotides and deoxyribonucleic acid.” Biochemistry 20: 5346-5352 PMID: 7028102
  2. Weiner JH et al (1975) “The deoxyribonucleic acid unwinding protein of Escherichia coli. Properties and functions in replication.” J. Biol. Chem. 250:1972-1980 PMID: 1090613

 

Fig. 1 SDS-PAGE of E. coli SSB protein

 

Fig. 2 Binding activity to single-stranded DNA

 

Related product:

Title Type Size
02-042, 02-042-5 E coli SSB_03222018 application/pdf 188 KB

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