Description
Description
HIV-1 Gag p17 is the matrix protein of HIV-1 virus and is processed by the digestion of its precursor Gag p55 by HIV-1 protease. This protein is indispensable to the reproduction of AIDS virus and constitute the essential element of the of AIDS virus particle construction (1).
The product is over-expressed as a recombinant protein in E. coli with a plasmid carrying the Gag p17 coding region of HIV-1 virus, subtype B (2), and highly purified by several steps of chromatography (3). Its molecular weight is 17 kD, same as that of p17 purified from HIV viral particles (Fig 1).
Usage
- It can be used as p17 antigen in detection of anti-HIV-1 p17 antibody in Western blotting or ELISA.
- It can be used as standard for the quantitative analysis of HIV-1 p17 antigen.
- It can be used in studies of structure and function of HIV-1 as matrix protein that constitutes HIV-1 core.
Specification
Purity: Over 90% by SDS-PAGE (CBB staining)
Protein concentration: 0.23 mg/ml as measured by BCA method
Form: 50% glycerol, 20mM Tris-HCl (pH7.5), 50mM NaCl, 10mM mercaptoethanol
Storage: -20oC
Reference
- Freed EO, Virology 251:1-15 (1998) Review
- Adachi A, et al., J. Virol. 59, 284 (1986)
- Saito A,et al., Microbiol. Immunol. 39:473-483 (1995)
Fig. 1 : Polyacrylamide gel electrophoresis of HIV-1 p17 protein
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