HIV-1 Gag p24

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SKU: 05-005 Research Area: , Applications:

Description

Description

HIV-1 Gag p24 is a capsid protein that constitutes the core of AIDS virus, HIV-1, and is produced by digestion of its precursor, Gag p55, by HIV-1 protease. This protein is indispensable for reproduction of AIDS virus and constitutes an essential element in the virus particle (1). As this protein is detectable from the early stage of AIDS virus infection, and reflects the amount of virus in the blood, it is used as a marker for observing the patient’s condition during and after treatment.

 

This protein was over-expressed as a recombinant protein in E. coli with a plasmid carrying the Gag p24 coding region of HIV-1 virus, subtype B (2), and highly purified by several steps of chromatography (3). Its molecular weight is 24 kD, same as that of p24 purified from HIV-1 virus particles (Fig 1).

 

Usage

1) It can be used as a standard in titration of p24 antigens

2) It can be used as antigen for Western blot or ELISA of anti-HIV-1 p24 antibody.

3) It can be used in the studies of structure and function of HIV-1 virus as it constitutes HIV-1 core as a capsid protein.

 

Specification

Purity: Over 90% purity by SDS-PAGE (CBB staining)

Protein concentration: 1 mg/ml measured by BCA method

Form: 50% glycerol, 20mM Tris-HCl (pH 7.5), 50mM NaCl, 10mM mercaptoethanol

Storage: -20°C

Data Link GenBank: AAA44988.1

 

References

  1. Freed EO Virology 251:1-15 (1998)
  2. Adachi A et al. J Virol 59: 284 -291(1986)
  3. Tanaka N et al. Microbiol Immunol 36: 823-831 (1992)
  4. Saito A et al. Microbiol Immunol 39:473-483 (1995)

Fig.1 Polyacrylamide gel electrophoresis of HIV-1 p24 protein

Title Type Size
05-005 HIV-1p24_03222018 application/pdf 180 KB

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