Keratinocyte Growth Factor (KGF), also known as Fibroblast Growth Factor 7 (FGF-7), is a member of fibroblast growth factor (FGF) family. Although FGF-7 has heparin binding activity similar to FGF-1, its mitogenic activity is predominantly exhibited in keratinocytes. It is not effective to fibroblasts and endothelial cells.
The human FGF-7 lacking the signal sequence (1-31 aa) was expressed in E. coli and purified by the chromatographic procedures. This product is an intact enzyme without tag with 19 kDa size (Fig. 1)
- Mitogen for epithelial cells
- Western blot control for anti-FGF-7 antibodies
- Acceleration of wound healing is implied.
- Acceleration of hair development is implied.
Activity: The ED50 as determined by a cell proliferation assay using MTS assay kit
(CellTiter 96, Promega) with human keratinocyte JCRB141 cells was < 10 ng/ml.
Purity: >95% as determined by SDS-PAGE (CBB staining)
Form: 1.0 mg / ml in PBS (10mM Na-phosphate, 150mM NaCl) pH7.2, 50% glycerol, filter-sterilized
Storage: -20°C or for long term storage -80°C
- Rubin JS et al. (1989) “Purification and characterization of a newly identified growth factor specific for epithelial cells.” Proc Natl Acad
Sci USA 86: 802-806 PMID: 2915979
- Aaronson SA et al. (1991) ”Keratinocyte growth factor. A fibroblast
growth factor family member with unusual target cell specificity.”
Ann NY Acad Sci 638:62-77 PMID: 1664700