Pfu DNA polymerase (with dNTPs)

$75.00

SKU: 02-021 Research Area: Applications:

Description

Pyrococcus furiosus DNA polymerase (Pfu DNA polymerase) gene was expressed in E. coli in large quantities and highly purified. The enzyme has thermostable DNA polymerase activity and 3’ →5’ exonuclease (proofreading) activity. The MW is 90 kDa, same as that of the natural Pfu DNA polymerase.

  • Pfu DNA polymerase is thermostabe and has low error rates.
  • It is suitable for PCR and primer extension reactions that require high fidelity synthesis.
  • Pfu DNA polymerase-generated PCR fragments are blunt-ended.

 

Applications

  1. Cloning
  2. DNA expression
  3. site-directed mutagenesis

 

Specification

Storage Conditions: 50mM Tris-HCl (pH 8.2), 0.1mM EDTA, 1mM DTT, 50% glycerol, 0.1% Tween20, 0.1% Igepal CA-630

Store at -20°C

Concentration: 2.5 units/ul, where one unit is defined as the amount of enzyme that can incorporate 10 nmols of dNTPs into an acid-insoluble material in 30 minutes at 72°C when activated salmon sperm DNA was used as template/primer.

 

Quality Assurance:

Greater than 95% of protein determined by SDS-PAGE (CBB staining) (Fig.1)

The absence of endonucleases and exonucleases was confirmed.

PCR Test: Good amplification result was obtained in PCR reaction using λDNA as a template (Fig. 2).

 

Reagents Supplied with Enzyme:

10 x Reaction Buffer (Pfu): 200mM Tris-HCl (pH 8.8), 100mM KCl, 100mM (NH4)2SO4, 20mM MgSO4, 1% TritonX-100, 1 mg/ml BSA

2.5mM (each) dNTPs

Fig. 1 SDS-PAGE of Pfu DNA polymerase

 

Fig. 2 Amplification of λ DNA

Title Type Size
02-021_03222018 application/pdf 226 KB

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