Rad52 Protein (Human)

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SKU: 10-003 Research Area: Applications:

Description

Human Rad52 protein plays a major role in genetic recombination and recombination repair by mediating strand-annealing reaction between complementary DNA strands (1). Rad52 functionally and physically interacts with Rad51, Dmc1, and RPA in recombination processes. In radiation damaged cell, Rad52 is phosphorylated at Tyr104 by c-Abl tyrosine kinase and forms nuclear foci (2).

The product was highly purified from E. coli over-expressing human Rad52 protein as a recombinant protein (3).

Applications

  • Studies on homologous recombination in mammals including human
  • Studies on the interaction of Rad52 protein with various proteins
  • To be used as a standard for Western Blot.

Specification

Purity: >90% as judged from SDS-PAGE analysis (Fig.1)

Form: 1.0 mg/ml in 20 mM potassium phosphate pH 7.5 200 mM KCl, 2 mM 2ME, 0.5 mM  EDTA, 20% glycerol

Storage: -70oC

Reference (This product was used in reference 3)

  1. Friedberg EC, et al. DNA Repair and Mutagenesis 2nd, ASM Press
  2. Kitao H & Yuan Z M, J. Biol. Chem. 277:48944 (2002)
  3. Kurumizaka H et al. J Biol Chem 276: 35201 (2001)

 

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Title Type Size
10-003 Rad52 (human)_03222018 application/pdf 174 KB

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