Our Osteoclast Culture Kit includes cryopreserved osteoclast precursor (OCP) cells from bone marrow and culture medium containing M-CSF and RANKL for researchers studying osteoporosis, bone reabsorption, or other bone metabolism disorders.?Osteoclast cells form from a hematopoietic stem cells called monocytes. Osteoclasts resorb bone by attaching to the bone surface and lowering the surrounding pH to an acidic level of around 4.5. The bone mineral is then solubilized and the collagen degraded. Osteoclast differentiation and function is controlled primarily by Macrophage Colony-Stimulating Factor (M-CSF) and Receptor for Activation of Nuclear Factor Kappa B Ligand (RANKL). The absence of NF-Kappa B leads to the bone disease osteopetrosis and the absence of M-CSF results in the arrest of differentiation in the pre-osteoclast stage. B-Bridge’s Osteoclast Culture Kit includes cryopreserved osteoclast precursor (OCP) cells from bone marrow and culture medium containing M-CSF and RANKL for researchers studying osteoporosis, bone reabsorbtion, or other bone metabolism disorders.
The two included 96-strip-well Osteoplates have a unique 3-dimensional structure that mimics in vivo bone for in vitro bone cell assays. This inorganic bone biomaterial surface in a multiple well plate is capable of supporting the functional properties of osteogenic cells. The assay surface is manufactured using a proprietary surface coating technology, which delivers lot-to-lot consistency, translating to consistent and reproducible results in bone cell assays. This surface also offers a consistent and defined alternative to preparing dentine or bone slices, reducing the variability in your assay system and resulting in more predictable assay readouts.