Description
ReverTra Ace™ qPCR RT Master Mix (Code No. FSQ-201) is a premix version of ReverTra Ace™ qPCR RT Kit. The master mix reagent (5x) contains the highly efficient reverse transcriptase “”ReverTra Ace™”” , primers and buffer optimized for highly efficient synthesis of short-chain cDNAs suitable for real-time PCR.
<FEATURES>
The optimized RT buffer and Primer Mix enable the highly efficient reverse transcription.
The reaction can be completed in 15 min. The protocol does not contain an additional RNase H treatment step to remove residual RNA after reverse transcription (Patent Pending).
Suitable for the detection of low-expressing mRNAs. Since the RT buffer is optimized for real-time PCR, the addition of 20% (v/v) of the synthesized cDNA solution to the PCR solution does not inhibit the PCR reaction.
<APPLICATIONS>
Reverse transcriptation for real-time PCR
ReverTra Ace™ qPCR RT Master Mix
5 x RT Master Mix 400 µL
5 x RT Master Mix no RT-Control 40 µL
Nuclease-free water 1000 µL × 2″