ReverTra Ace™ qPCR RT Master Mix (Code No. FSQ-201) is a premix version of ReverTra Ace™ qPCR RT Kit. The master mix reagent (5x) contains the highly efficient reverse transcriptase “”ReverTra Ace™”” , primers and buffer optimized for highly efficient synthesis of short-chain cDNAs suitable for real-time PCR.
The optimized RT buffer and Primer Mix enable the highly efficient reverse transcription.
The reaction can be completed in 15 min. The protocol does not contain an additional RNase H treatment step to remove residual RNA after reverse transcription (Patent Pending).
Suitable for the detection of low-expressing mRNAs. Since the RT buffer is optimized for real-time PCR, the addition of 20% (v/v) of the synthesized cDNA solution to the PCR solution does not inhibit the PCR reaction.
Reverse transcriptation for real-time PCR
ReverTra Ace™ qPCR RT Master Mix
5 x RT Master Mix 400 µL
5 x RT Master Mix no RT-Control 40 µL
Nuclease-free water 1000 µL × 2″