This product is a 2 × Master Mix for “”1-step real-time PCR”” using a thermostable DNA polymerase derived from Thermus thermophilus (Tth) HB8 (1). Tth DNA polymerase exhibits reverse transcriptase activity in the presence of Mn2+ ions. This system allows for “”1-step real-time PCR””, including reverse transcription and PCR steps. RNA-direct™ Realtime PCR Master Mix is applicable for TaqMan™ assay or hybridization probe assay, in combination with each probe.
Suitable for high-throughput real-time PCR and increases reliability of product, due to lowered risk of contamination.
Can be used in systems using glass capillaries (e.g., LightCycler, Roche Molecular Systems, Inc.).
Can be used in systems using passive reference (e.g., ABI PRISM™ 7700, Applied Biosystems, Inc.).
Hot Start technology, using anti-Tth DNA polymerase antibodies, allows for high specificity and reproducible amplification.
This reagent includes the following components for 100 reactions, with a total of 50 µL per reaction. All reagents should be stored at -20ºC.
RNA-direct™ Real-time PCR Master Mix 0.5 mL x 5
50 mM Mg(OAc)2 0.5 mL x 1