This product is a recombinant type human ribonuclease inhibitor purified using a combination of ion exchange and affinity chromatography.
This inhibitor exhibits broad-spectrum RNase inhibitory properties, including RNase A, RNase B, RNase C and human placental RNase. Does not inhibit RNase T1, S1 nuclease, RNase from Aspergillus, and RNase H. The 50kDa protein exerts its inhibitory effect by noncovalently binding to RNases in a 1:1 ratio. This inhibitor can be applied to a reverse transcriptase reaction.
RNase inhibitor (20-40 U/µL)*
Stabilizes RNA in a cDNA synthesis or a RNA synthesis reaction.
Dees not inhibit SP6, T7 or T3 RNA Polymerase, AMV or M-MLV Reverse Transcriptase, or Taq DNA polymerase.
Exhibits broad-spectrum RNase inhibitory properties.
Active over a broad pH range (pH 5-8).
cDNA synthesis, in vitro transcription, in vitro translation
* Specific activity : approximately 100 U/µg protein