Description
AS ONE SuperHiFi DNA Polymerase 2x Master Mix is a ready-to-use 2x reaction mix which includes AS ONE SuperHiFi DNA Polymerase in an optimized buffer system with dNTPs and magnesium chloride. The 2x Master Mix allows robust amplification on DNA target with low to high GC content and long DNA targets.
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Cat. No. | Size
Reactions |
SuperHiFi High Fidelity DNA Polymerase 2x Master Mix 4 mM MgCl2 |
AO620701 | 100 | 2 x 1.25 ml |
AO620703 | 500 | 10 x 1.25 ml |
AO620706 | 2500 | 50 x 1.25 ml |
AO620707 | 5000 | 25 x 5 ml |
Key Features
- Convenient reaction set-up
- High fidelity – measured up to 50x Taq fidelity
- Excellent coverage for amplification of difficult amplicons with low to high GC content
- Long range capability: 8.5 kb for gDNA and ≤ 5 kb for λDNA
- Recommended for cloning, mutagenesis and other molecular applications requiring extremely high fidelity
SuperHiFi High Fidelity DNA Polymerase 2x Master Mix is an all-in-one 2x master mix containing the SuperHiFi High Fidelity DNA Polymerase, SuperHiFi Buffer, dNTPs and MgCl2. Simply mix SuperHiFi High Fidelity DNA Polymerase 2x Master Mix with primers, DNA template and water and you are ready to carry out PCR.
SuperHiFi High Fidelity DNA Polymerase is a thermostable DNA Polymerase with proofreading ability. SuperHiFi High Fidelity DNA Polymerase exhibits 5’→3’ DNA polymerase activity and 3’→5’ proofreading exonuclease activity. The latter allows the enzyme to correct mis-incorporated nucleotides. The SuperHiFi High Fidelity DNA Polymerase 2x Master Mix exhibits robust amplification of targets with low to high GC content, as well as a fidelity* up to 50x Taq.
*Estimated by NGS technology using the Illumina MiSeq instrument.