SuperHiFi High Fidelity DNA Polymerase with 10x Buffer


SKU: AO61040 Research Area: Applications:


AS ONE SuperHiFi Polymerase is a proof-reading DNA polymerase with the ability to perform robust amplification of a vast range of difficult targets, including those up to 12.5 kb and with high to low GC content. The fidelity of the polymerase has been measured up to 50x Taq DNA Polymerase. The features of AS ONE SuperHiFi Polymerase have been attained by combining functional domains from two wildtype Archaeal high fidelity DNA polymerases, thereby creating a unique and chimeric DNA Polymerase displaying the most desired features from both wildtype DNA Polymerases. AS ONE SuperHiFi DNA Polymerase exhibits both 5’→3′ DNA polymerase activity and 3’→5′ proofreading exonuclease activity enabling this polymerase to correct base pair mismatches. AS ONE SuperHiFi is well suited for PCR experiments whose outcome is strictly dependent on amplifications with very low error rates, such as cloning/sub-cloning, NGS applications and mutagenesis.


Also interested in SuperHiFi Master Mixes? Click here.



Cat. No.




DNA Polymerase

10x SuperHiFi Buffer

20 mM MgCl2

Mg Cl2

25 mM

AO610401 100 50 ul 1.5 ml 1.5 ml
AO610403 500 250 ul 2 x 1.5 ml 1.5 ml
AO610404 1000 2 x 250 ul 4 x 1.5 ml 2 x 1.5 ml
AO610406 2500 5 x 250 ul 9 x 1.5 ml 5 x 1.5 ml


Key Features

  • High fidelity – measured up to 50x Taq fidelity
  • Excellent coverage for amplification of difficult amplicons with low to high GC content
  • Longrange capability: 5 kb for gDNA and ≤ 12.5 kb for λDNA
  • Recommended for cloning, mutagenesis and other molecular applications requiring extremely high fidelity


SuperHiFi High Fidelity DNA Polymerase is a thermostable DNA Polymerase with proofreading ability. SuperHiFi High Fidelity DNA Polymerase exhibits 5’→3’ DNA polymerase activity and 3’→5’ proofreading exonuclease activity. The latter allows the enzyme to correct mis-incorporated nucleotides. By using the 10x SuperHiFi Buffer the polymerase exhibits robust amplification of targets with low to high GC content, as well as a fidelity* up to 50x Taq.


*Estimated by NGS technology using the Illumina MiSeq instrument.


Title Type Size
AO61040X data sheet application/pdf 250 KB

Additional Information