T4 Gene 32 Protein (Single-stranded DNA Binding Protein, SSB)

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SKU: 02-040 Research Area: Applications:

Description

T4 gene 32 protein is a single-stranded DNA binding protein from phage T4 which binds to single-stranded DNA with high specificity (1, 2). It is involved in DNA replication and recombination. The T4 phage-derived SSB gene was expressed in E. coli and the protein was highly purified. MW is 33.5 kDa.

 

Applications

  1. Promoting DNA replication and recombination by stabilizing single-stranded DNA (1)
  2. Increase specificity and yields of long PCR products (3)

 

Specification

Quality: Greater than 95% of protein determined by SDS-PAGE (CBB staining). The absence of endonucleases and exonucleases was confirmed.

Form: 10 mg/ml in 20mM Tris-HCl (pH 8.0), 100mM NaCl, 0.5mM dithiothreitol, 1mM EDTA, 50% glycerol

Storage: -20°C

 

Data Link: UniProtKB/Swiss-Prot P03695 (VHED_BPT4)

 

References:

  1. Alberts BM & Frey L (1970) “T4 bacteriophage gene 32: a structural protein in the replication and recombination of DNA”. Nature 227:1313-1318 PMID:5455134
  2. Bittner M et al (1979) “Purification of the T4 gene 32 protein free from detectable deoxyribonuclease activities” J Biol Chem 254: 9565-9572 PMID:226522
  3. Schwarz K et al (1990) ”Improved yields of long PCR products using gene 32 protein” Nucleic Acids Res 18:1079 PMID:2107527

Fig. 1 SDS-PAGE of T4 SSB protein

 

Fig. 2 Binding activity to single-stranded DNA

 

Related product:

Title Type Size
02-040, 02-040-5 T4 Gene 32 Protein_03222018 application/pdf 183 KB

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