Thermus aquaticus Single-stranded DNA Binding Protein (SSB)

$200.00

SKU: 02-044 Research Area: Applications:

Description

Thermus aquaticus derived single-stranded DNA binding protein (SSB) is a thermostable protein which binds to single-stranded DNA with high specificity but does not bind well to double-stranded DNA (1). It plays important roles in DNA replication and recombination (2). Thermus aquaticus SSB gene was expressed in E. coli in large quantities and the protein was purified. MW is 30.0 kDa, same as that of the natural protein.

 

Application

Stabilizes single-stranded DNA in DNA replication, repair, and recombination

 

Specification

Concentration: 1.0 mg/ml in 50mM Tris-HCl (pH 8.0), 200mM NaCl, 0.1mM dithiothreitol, 0.5mM EDTA, 50% glycerol

Store at -20°C

Activity: Single-stranded DNA binding activity was confirmed (Fig.2).

Quality: Greater than 95% of protein determined by SDS-PAGE (CBB staining). The absence of endonucleases and exonucleases was confirmed.

 

Data Link: UniProtKB/Swiss-Prot Q9KH06 (SSB_THEAQ)

 

References:

  1. Dabrowski S et al (2002) “Novel thermostable ssDNA-binding proteins from Thermus thermophilus and T. aquaticus-expression and purification.” Protein Expr Purif 26: 131-138 PMID: 12356480
  2. Greipel J et al (1989) In Saenger, W. and Heinemann, U. (eds), Protein-Nucleic Acid Interaction, Macmillan, London, pp.61-86

Fig. 1 SDS-PAGE of Thermus aquaticus SSB

 

Fig. 2 Binding activity to single-stranded DNA

 

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Title Type Size
02-044 Thermus aquaticus SSB_03222018 application/pdf 199 KB

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