Tartrate-resistant acid phosphatase staining of osteoclasts
This New TRAP Staining Kit (cat. #PMC-AK04FN-COS) is used for the staining of tartrate-resistant acid phosphatase in osteoclasts. Bone mass is controlled by the balance between the activity of osteoblasts and the activity of osteoclasts. Alkaline phosphatase and tartrate-resistant acid phosphatase are used as markers for osteoblasts and osteoclasts, respectively. The TRAP Staining Kit supplements the Bone Reabsorption Assay Kits.
The TRAP Staining Kit uses the same buffer as Acid Mucopolysaccharide Assay and DNA Quantity Assay, therefore a single sample can be shared among these assays.
One kit contains reagents for staining 10 x 96-well plates.
▪ Fixative, 10% Formalin, neutral buffer (Reagent 1) 1 bottle, 60 mL
▪ Tartrate-containing Buffer, 50 mM, pH 5.0 (Reagent 2) 1 bottle, 50 mL
▪ Chromogenic Substrate (Reagent 3) 10 vials, 3 mg/vial