anti-Cnd2 (fission yeast) Antibody, Rabbit Serum

Description

Cnd2 protein is a regulatory subunit of the condensin complex, which is required for conversion of interphase chromatin into mitotic condensed chromosomes in S. pombe. The condensin complex also plays a role during interphase in processes of DNA repair and recombination.

 

Although molecular mass of the Cdn2 protein of S. pombe from the DNA sequence is 83.2 kDa, it migrates on SDS-PAGE at a position equivalent to a 105 kDa protein (Ref. 2).

 

Application

Western blot (1:500 dilution) (Ref.1,2,3)

Immunofluorescent staining (Ref. 3)

Not tested for other applications

 

Specification

Immunogen: Recombinant GST-Cnd2 fusion protein expressed in E. coli

Specificity: Reacts with S. pombe Cnd2 protein. Not tested for other species

Form: Rabbit antiserum with 0.05 % sodium azide

Storage: Ship 4°C and long term storage -20°C

 

Data Link

cnd2: pombe : GeneDB

 

References: This antibody was used in the following references.

Chen ES, Sutani T, Yanagida M. Chen ES, Sutani T, Yanagida M.（2004 Cti1/C1D interacts with condensin SMC hinge and supports the DNA repair function of condensin. Proc Natl Acad Sci U S A. 101:8078-83. PNAS Content

 

Aono N, Sutani T, Tomonaga T, Mochida S, Yanagida M.（2002） Cnd2 has dual roles in mitotic condensation and interphase. Nature. 417:197-202. pubmed/12000964

 

Morishita J.(2001) Bir1/Cut17 moving from chromosome to spindle upon the loss of cohesion is required for condensation, spindle elongation and repair. Genes Cells. 6:743-63. Genes to Cells – 　Wiley Online Library

 

Fig. 1. Identification of Cnd2 protein in the crude extract of S. pombe by western blot with this antiserum.

 

 

Fig.2 Detection of Cnd2 protein by immunofluorescent staining of S. pombe cell.

 

 

Fig.3 Identification of Cnd2 protein and Cnd2-GFP protein in crude cell extract by anti-Cnd2 antibody at 1/500 dilution.