anti-GST antibody, rabbit serum

Description

Glutathione S transferase (GST) from Schistosoma japonicum is commonly used to create fusion proteins. GST-tag has the size of 220 amino acids (roughly 26kDa) and is fused to the N-terminus of a protein. GST fusion proteins can be produced in Escherichia coli, as recombinant proteins and are used to purify and detect proteins of interest. The GST part binds its substrate, glutathione. GST-fusions protein can be easily purified from cell extracts by affinity chromatography with glutathione resin.

 

Applications

1. Western blot dilution: 1/2,000~1/10,000
2. Immunoprecipitation assay dependent
3. ELISA

Other applications have not been tested

 

Specification

Immunogen: Recombinant full-size GST (1-212 aa)

Form: Antiserum with 0.05% sodium azide

Specificity Specific to GST and GST-tagged proteins

Storage: -20°C or for long term storage, -70°C

 

Data Link

NCBI Protein Data AAA57089

 

References

1. Smith DB & Johnson KS (1988) “Single-step purification of polypeptides expressed in Escherichia coli as fusions of glutathione-S-transferase.” Gene 67:31-40 PMID: 3047011
2. Kaelin WG Jr et al (1991) “Identification of cellular proteins that can interact specifically with the T/E1A-binding region of the retinoblastoma gene product.” Cell 64:521-532 PMID: 1825028
3. Molecular Cloning: A laboratory Manual (eds. Sambrook, J., Russell, D.W. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, USA, 2001) pp.15.36-15.39, pp.18.48-18.59.

Fig.1 Detection of GST-tagged protein with this antibody by Western blotting.

-: Lysate of 293T cells transfected with an empty vector

: Lysate of 293T cells transfected with the plasmid carrying

the GST-tagged importin gene

Fig.2 Immunoprecipitation of GST-tagged protein with this antibody followed by Western blot.

-: Lysate of 293T cells transfected with an empty vector

: Lysate of 293T cells transfected with the plasmid carrying

the GST-tagged INbox gene