Anti-RBP2/JARID1A antibody, mouse monoclonal (9A6)

Description

RBP2 was originally identified as a retinoblastoma binding protein. It is also known as JARID1A (Jumonji, AT rich interactive domain 1A). RBP2 plays both negative and positive roles in RB-mediated transcriptional activation, depending on the kinds of genes and regulates differentiation by its function as an H3K4 histone demethylase (1, 2 & 3).

Applications

Western blot ~1 µg/ml

Other applications were not tested.

Specification

Immunogen: A synthetic peptide corresponding to human RBP2, amino acids 1416-1434.

Isotype: Mouse IgG2a kappa

Form: Purified monoclonal antibody (IgG) 1mg/ml in PBS, 50% glycerol, filter-sterilized

Specificity: Specific to human and mouse RBP2. Can detect endogenous levels of RBP2.

Storage: -20°C, for long term storage -70°C

Data Link: UniProtKB/Swiss-Prot P29375 (KDM5A_HUMAN)

References

1. Lopez-Bigas N et al “Genome-wide analysis of the H3K4 histone demethylase RBP2 reveals a transcriptional program controlling differentiation” Moll Cell 31: 520-530 (2008) PMID: 18722178
2. Klose RJ et al “The retinoblastoma binding protein BRP2 is an H3K4 demethylase” Cell 128: 889-900 (2007) PMID: 17320163
3. Christensen J et al “RBP2 belongs to a family of demethylases, specific for tri- and dimethylated lysine 4 on histone 3” Cell 128:1063-1076 (2007) PMID: 17320161

Figure: Identification of RBP2 in crude cell extracts by Western blot with antibody 9A6.

Lane: 1. HeLa control siRNA

Lane 2. HeLa RBP2 siRNA

Lane 3. MCF7

Lane 4. U2OS

Lane 5. NIH3T3

Lane 6. J1 (mouse ES)