Description
Background:
Rhp51 protein of Schizosaccharomyces pombe (fission yeast) is a functional and structural homolog of E.coli RecA protein and Rad51 proteins of eukaryotes, which play a major role in genetic recombination and recombination repair by mediating strand exchange reaction between homologous DNA strands.
Specifications:
Reactivity: Schizosaccharomyces pombe. Specificity has been validated with rhp51 mutant for WB (Fig.1)
Immunogen: Purified recombinant full-length Rhp51 protein
Form: Rabbit antiserum added with 0.09 % sodium azide
Storage temmperature: Shipped at 4℃ and store at -20℃
Applications:
- Western blotting (1/ 2,000~1/5,000) Fig.1
- Immunoprecipitation (1/100-1/500)
- Chromatin Immuno-Precipitation (Assay dependent)
- Immunofluorescence staining (1/500 dilution). Fig. 2
Data Link UniProtKB/Swiss-Prot P36601 (RAD51_SCHPO)
Fig.1 Western blot analysis of Rhp51 in the whole cell extracts.
M: Molecular size markers (kD)
Lane 1: Wild-type strain
Lane2: Rhp51 deletion mutant strain
Note that Rhp51 band is detected in lane 1, wild-type extract, but is absent in lane 2, rhp51 mutant extract.
Fig. 2 Rhp51 foci formation observed after DNA damage: S. pombe cells without or with MMS (0.025%) treatment for 1 h were processed for indirect immunofluorescence staining with anti-Rhp51 antibody (1/500 dilution). The Rhp51 foci formation is induced by DNA-damage treatment with MMS.
References: This product has been used in the following publications
1. Akamatsu Y et al. Two different Swi5-containing protein complexes are involved in mating-type switching and recombination repair in fission yeast. Proc Natl Acad Sci U S A. 2003 Dec 23;100(26):15770-5. WB, IP (S. pombe)
2. Kibe T et al. Fission yeast Rhp51 is required for the maintenance of telomere structure in the absence of the Ku heterodimer. Nucleic Acids Res.2003 Sep 1;31(17):5054-63. ChIP (S. pombe)
3. Lambert S et al “Gross chromosomal rearrangements and elevated recombination at an inducible site-specific replication fork barrier” Cell 121: 689-702 (2005) PMID: 15935756 IF (S. pombe)
4. Morishita T et al” Role of the Schizosaccharomyces pombe F-Box DNA helicase in processing recombination intermediates” Mol Cell Biol 25: 8074-8083 (2005) PMID: 16135799 IF (S.pombe)
5. Haruta N et al “The Swi5-Sfr1 complex stimulates Rhp51/Rad51-and Dmc1-mediated DNA strand exchange in vitro”Nat Struc Mol Biol 13: 823-830 (2006) PMID: 16921379 WB, IP (S. pombe)
6. Akamatsu Y et al. Fission yeast Swi5/Sfr1 and Rhp55/Rhp57 differentially regulate Rhp51-dependent recombination outcomes. EMBO J.2007 Mar 7;26(5):1352-62. IF (S. pombe)
7. Polakova S et al. Dbl2 Regulates Rad51 and DNA Joint Molecule Metabolism to Ensure Proper Meiotic Chromosome Segregation. PLoS Genet.2016 Jun 15;12(6):e1006102. IF (S. pombe)
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