E.coli RuvC Protein

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SKU: 01-011 Research Area: Applications:

Description

Background:

E. coli RuvC protein (19 kDa) is a structurally specific endonuclease which binds specifically to the Holliday structure, an intermediate of recombination, at the late stage of homologous recombination and recombination repair and introduces a nick in the symmetrical point of the Holliday junction cleaving and resolving the recombinant (1, 2).

 

Specifications:

 

Product: Recombinant E. coli full-size RuvC protein without tag.

Form: 50% glycerol, 10 mM Tris-HCl (pH7.5), 2 mM EDTA, 100 mM NaCl, 5 mM mercaptoethanol

Concentration: 1.0 mg/ml (determined by BCA method)

Storage: Sent at 4℃or at -20℃ and store at -20℃ or -80℃ for longer period.

 

Applications:

  • Functional studies in vitro. RuvC cleaves recombination

intermediate at Holliday Junction.

  • Standard antigen for western blotting and ELISA.
  • SDS-PAGE

 

Data Link UniProtKB/Swiss-Prot P0A814 (RUVC_ECOLI)

 

Figure. SDS-PAGE analysis of the purified RuvC protein. 19 kDa

 

References:  This product was used in Ref. 2 and 3.

  1. Shinagawa H and Iwasaki H (1996) “Processing the holliday junction in homologous recombination.” Trend Biochem Sci 21:107-111 PMID: 8882584
  2. Iwasaki H et al (1991) “Escherichia coli RuvC protein is an endonuclease that resolves the Holliday structure.” EMBO J 10:4381-4389 (1991) PMID: 1661673
  3. Murayama Y et al (2008) “Formation and branch migration of Holliday junctions mediated by eukaryotic recombinases.”Nature 451:1018-1021PMID:18256600

 

Related Products:

01-007 E.coli RuvA protein    01-009 E.coli RuvB protein  61-005 anti-RuvA antibody

61-007 anti-RuvB antibody    61-009 anti-RuvC antibody

Title Type Size
01-011 RuvC_03212018 application/pdf 284 KB

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