Description
Thermo T7 RNA polymerase is a genetically modified T7 RNA polymerase exhibiting increased thermal stability. The optimum reaction temperature of this enzyme is around 50ºC. The half-life of the enzyme at 50ºC is approximately 85 min.
<FEATURES>
Exhibits greater specific activity than WT-enzyme at 37-50ºC.
<APPLICATIONS>
RNA probe preparation
RNA synthesis for in vitro translation
RNA synthesis for RNA splicing studies
Capped mRNA synthesis using a cap analogue
<COMPONENTS>
Thermo T7 RNA polymerase (50U/µL)
10×Buffer”