Watch Video:

How Does Gravite Work?

 

Latest published studies that used Gravite (full-text PDFs are available upon request):

Furukawa T, Tanimoto K, Fukazawa T, Imura T, Kawahara Y, and Yuge L, Simulated microgravity attenuates myogenic differentiation via epigenetic regulations, NPJ Microgravity. 2018; 4: 11.

Otsuka T, Imura T, Nakagawa K, Shrestha L, Takahashi S, Kawahara Y, Sueda T, Kurisu K, Yuge L, Simulated microgravity culture enhances the neuroprotective effects of human cranial bone-derived mesenchymal stem cells in traumatic brain injury. Stem Cells Dev. 2018 Jul 5.

Imura T, Nakagawa K, Kawahara Y, Yuge L, Stem cell culture in microgravity and its application in cell-based therapy. Stem Cells Dev. 2018 Aug 7.

 A Microgravity Simulator that NASA Kennedy Space Center introduced!

Microgravity provides a special environment where cells grow without sedimentation and convection. Several studies have shown that the formation of 3D aggregates after the cells were cultured under microgravity conditions. The 3D multicellular spheroids or tissues represent more physiologically relevant in vivo situations needed for biomedical research and drug development.

Continue reading Gravity Controller Gravite®

Cell Stretching Systems

Menicon’s ShellPa and ShellPa Pro high-performance mechanical cell strain instruments and cell culture chambers allow researchers to explore the impact of mechanical cell strain on cells in culture. Mechanical stress applied to the cells better simulates a natural dynamic physiological environment.

• ShellPa Mechanical Cell Strain Instrument
• ShellPa Pro Mechanical Cell Strain Instrument

Use ShellPa and ShellPa Pro to investigate:

• Bone, muscle, cartilage, ligaments & tendons
• Cardiovascular system
• Cell adhesion, division, growth & cytoskeletal rearrangement
• Stem cell proliferation & differentiation

 

ShellPa Mechanical Cell Strain Instrument

The mechanical stresses are closely related to cell proliferation and differentiation or tissue degeneration and development. Additionally, disease processes such as osteoporosis, cancer metastasis, skin disorders and muscle degeneration share a loss of tissue structure, integrity and mechanical transduction. ShellPa, the mechanical cell stretch system from Menicon Life Science, is an instrument for cell culturing that has been developed to replicate a dynamic environment inside the human body. This system is pneumatically driven by an air compressor. It is expected that cell culturing under the mechanical stress provided by this system can bring more biologically and physiologically realistic cell responses compared to a conventional static cell culturing without mechanical stress.

Features:

• The stretch frequency and stretch ratio (stretch pattern) are easily controllable.
• Stretch chambers are made with silicone (polydimethylsiloxane, or PDMS) and can be used with this system after being coated (e.g. fibronectin).
• Lightweight and compact
• Fits in standard laboratory incubator
• Stretch frequency: 1-120 cycle(s)/min
• Stretch ratio: 2, 4, 5, 6, 8, 10, 12, 15 and 20%
• Use up to 6 stretch chamber simultaneously
• Removable stretch chambers holder
• Controller clearly indicates the accumulated operation time

Download : Brochure for ShellPa System

Recent publications:

• Gangoda, S., et al. “Pulsatile stretch as a novel modulator of amyloid precursor protein processing and associated inflammatory markers in human cerebral endothelial cells“, Scientific Reports, volume 8, Article number: 1689 (2018)
• Grave, K., et al. “ANGIOTENSIN-(1-7) IMPROVES ENDOTHELIAL FUNCTION AND THE DEVELOPMENT OF ATHEROSCLEROSIS THROUGH THE MAS RECEPTOR.”Journal of Hypertension 34 (2016): e24.
• Dalbeni, A., et al. “LYCOPENE INCREASES NITRIC OXIDE BIOAVAILABILITY AND INHIBITS ENDOTHELIAL CELLS MIGRATION.”Journal of Hypertension 34 (2016): e24.
• Gangoda, S. V. S., et al. “PULSATILE STRETCH ALTERS EXPRESSION AND PROCESSING OF AMYLOID PRECURSOR PROTEIN IN HUMAN CEREBRAL ENDOTHELIAL CELLS.”Journal of Hypertension 34 (2016): e24.
• Urbanski, K., et al. “PERIVASCULAR T REGULATORY CELLS AND ENDOTHELIAL DYSFUNCTION IN HUMAN ATHEROSCLEROSIS.”Journal of Hypertension 34 (2016): e24-e25.
• Thal, Serge C., and Winfried Neuhaus. “The blood–brain barrier as a target in traumatic brain injury treatment.”Archives of medical research 8 (2014): 698-710.

ShellPa Cell Strain Products:

Cat #	Name	Size
STMP	Programmable ShellPa Pro mechanical cell strain instrument	Main Unit, Controller
NNMS	Standard ShellPa mechanical cell strain instrument	Main Unit, Controller, Air Compressor
SC4Dea	4cm2 Stretch Chamber	Pack of 6 chambers

 

AS ONE INTERNATIONAL is proud to offer Histofine IHC (Immunohistochemistry) detection kits for human, mouse, rabbit, and goat primary antibodies. Uniquely, these kits use just the antigen-recognizing Fab fragment (rather than a whole antibody) to detect a primary antibody. This feature minimizes non-specific binding, thereby increasing the signal-to-noise ratio and generating cleaner results.

Continue reading IHC Detection

AS ONE INTERNATIONAL is proud to bring you two brands of distinct hydrogels for 3-D cell culture: Cellendes, a life science company in Germany, developed a biomimetic dextran-based hydrogel while Menicon Life Science in Japan manufactures a peptide-based hydrogel. Each hydrogel offers unique advantages for a variety applications like drug discovery and tissue engineering.

Continue reading 3-D Cell Culture Hydrogels

Are you tired of finding restriction sites? AS ONE INTERNATIONAL provides high quality and easy-to-use kits and reagents for cloning, sub-cloning, generating transgenic animals, and manipulating DNA constructs. Particularly, Red/ET Recombineering offers unprecedented freedom in gene cloning. It gives you the powerful tools to clone, subclone, and modify DNA at any chosen position. These Red/ET Kits, Selection Cassettes, and Recombinase Plasmids permit precise engineering of DNA molecules of any size, including very large ones such as BACs or the E. coli chromosomes. Continue reading Red/ET Recombineering

AS ONE INTERNATIONAL is a leading supplier of the assay kits for metabolism, diabetes and obesity research. You can find our well-referenced Adiponectin, Leptin, Resistin, and esRAGE ELISA kits, which provide a broad linear detection range, picograms per milliliter sensitivity, and highly specific antibodies and give you consistent and reliable colorimetric readings. In addition, our caspases assay kits, cathepsins assay kits and FLISP kits would help advance your apoptosis studies.

Continue reading Featured Assay Kits